Recombinant human Mimitin/NDUFAF2 protein

NDUFAF2, also known as Mimitin, belongs to the complex I NDUFA12 subunit family. NADH dehydrogenase is an enzyme located in the inner mitochondrial membrane that catalyzes the transfer of electrons from NADH to coenzyme Q (CoQ) . It is the 'entry enzyme' of oxidative phosphorylation in the mitochondria. Mimitin protein acts as a molecular chaperone for mitochondrial complex I assembly. Recombinant human NDUFAF2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2, Mimitin, MMTN, NDuFA12L, NADH:ubiquinone oxidoreductase complex assembly factor 2, B17.2-like, B17.2L, Myc-induced mitochondrial protein, NDUFA12-like protein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

22 kDa (189aa) confirmed by MALDI-TOF

Additionnal Information

NDUFAF2, Mimitin, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2, Mimitin, MMTN, NDuFA12L, ATGP1133-10 µg, ATGP1133-20 µg, ATGP1133-50 µg, ATGP1133-100 µg, ATGP1133-250 µg, ATGP1133-500 µg, ATGP1133-1 mg, ATGP1133-10, ATGP1133-20, ATGP1133-50, ATGP1133-100, ATGP1133-250, ATGP1133-500, ATGP1133-1

References & Citations

Tsuneoka M., et al (2005) J. Biol. Chem. 280:19977-19985; ; Ogilvie I., et al (2005) J. Clin. Invest. 115:2784-2792

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 200 mM NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_777549

Uniprot Accession Number

Q8N183

Species

Human

AA Sequence

MGWSQDLFRA LWRSLSREVK EHVGTDQFGN KYYYIPQYKN WRGQTIREKR IVEAANKKEV DYEAGDIPTE WEAWIRRTRK TPPTMEEILK NEKHREEIKI KSQDFYEKEK LLSKETSEEL LPPPVQTQIK GHASAPYFGK EEPSVAPSST GKTFQPGSWM PRDGKSHNQ