Recombinant e.coli ppa protein
Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations. Recombinant E. coli ppa protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Inorganic pyrophosphatase, ECK4222, JW4185
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
21.9 kDa (196aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Harold FM., et al (1966) Bacteriol Rev 30 (4) : 772-94.; ; Carman GM., et al (2006) Trends Biochem. Sci. 31 (12) : 694-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_418647
Uniprot Accession Number
P0A7A9
Species
E.coli
AA Sequence
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