Recombinant human TFF1 protein
TFF1 (Trefoil factor 1) is member of trefoil family, characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. TFF1 has also been detected in a number of carcinomas including cancers of the breast, pancreas and stomach. Recombinant human TFF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Trefoil factor 1, pS2, BCEI, HPS2, HP1.A, pNR-2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
9 kDa (81aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
May FE., et al. (1997) . J. Pathol. 183: 4-7.; ; Gott P, et al. (1997) . Eur J Hum Genet 4 (6) : 308-15.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100 mM NaCl, 0.1mM PMSF
Scientific Category
Growth factors
NCBI Accession Number
NP_003216
Uniprot Accession Number
P04155
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MEAQTETCTV APRERQNCGF PGVTPSQCAN KGCCFDDTVR GVPWCFYPNT IDVPPEEECE F
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