Recombinant human SHC1 protein

SHC1 (SHC-transforming protein 1) belongs to the SH2 domain family. SHC1 has been found to be important in the regulation of apoptosis and drug resistance in mammalian cells. It is a signaling adapter that couples activated growth factor receptors to signaling pathway. SHC1 expressed in neural stem cells but absent in mature neurons. Recombinant human SHC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

SHC-transforming protein 1, FLJ26504, SH2 domain protein C1, SHC, SHC-transforming protein 3, p66, MGC98812, SHCA, Src homology 2 domain containing transforming protein C1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

53.8 kDa (494aa)

Additionnal Information

SHC1, SHC-transforming protein 1, FLJ26504, SH2 domain protein C1, SHC, SHC-transforming protein 3, p66, MGC98812, SHCA, Src homology 2 domain containing transforming protein C1, ATGP1128-10 µg, ATGP1128-20 µg, ATGP1128-50 µg, ATGP1128-100 µg, ATGP1128-250 µg, ATGP1128-500 µg, ATGP1128-1 mg, ATGP1128-10, ATGP1128-20, ATGP1128-50, ATGP1128-100, ATGP1128-250, ATGP1128-500, ATGP1128-1

References & Citations

Debnath J., et al. (2010) Oncogene. 29 (41) :5556-8.; ; Zhou M.-M., et al. (1995) Proc. Natl. Acad. Sci. u.S.A. 92:7784-7788

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl, 0.1mM PMSF

Scientific Category

Neuroscience

NCBI Accession Number

NP_003020

Uniprot Accession Number

P29353

Species

Human

AA Sequence

MNKLSGGGGR RTRVEGGQLG GEEWTRHGSF VNKPTRGWLH PNDKVMGPGV SYLVRYMGCV EVLQSMRALD FNTRTQVTRE AISLVCEAVP GAKGATRRRK PCSRPLSSIL GRSNLKFAGM PITLTVSTSS LNLMAADCKQ IIANHHMQSI SFASGGDPDT AEYVAYVAKD PVNQRACHIL ECPEGLAQDV ISTIGQAFEL RFKQYLRNPP KLVTPHDRMA GFDGSAWDEE EEEPPDHQYY NDFPGKEPPL GGVVDMRLRE GAAPGAARPT APNAQTPSHL GATLPVGQPV GGDPEVRKQM PPPPPCPAGR ELFDDPSYVN VQNLDKARQA VGGAGPPNPA INGSAPRDLF DMKPFEDALR VPPPPQSVSM AEQLRGEPWF HGKLSRREAE ALLQLNGDFL VRESTTTPGQ YVLTGLQSGQ PKHLLLVDPE GVVRTKDHRF ESVSHLISYH MDNHLPIISA GSELCLQQPV ERKL