Recombinant human ATF3 protein

ATF3 (activating transcription factor 3) is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription, it is presumed, by sequestering inhibitory co-factors away from the promoter.

Product Specifications

Product Name Alternative

Activating transcription factor 3, ATF3deltaZip3, cAMP dependent transcription factor ATF3, Cyclic AMP dependent transcription factor ATF3, Cyclic AMP-dependent transcription factor ATF-3

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

20.6 kDa (181aa)

Additionnal Information

ATF3, Activating transcription factor 3, ATF3deltaZip2, ATF3deltaZip2c, ATF3deltaZip3, cAMP dependent transcription factor ATF3, Cyclic AMP dependent transcription factor ATF3, Cyclic AMP-dependent transcription factor ATF-3, ATGP1127-10 µg, ATGP1127-20 µg, ATGP1127-50 µg, ATGP1127-100 µg, ATGP1127-250 µg, ATGP1127-500 µg, ATGP1127-1 mg, ATGP1127-10, ATGP1127-20, ATGP1127-50, ATGP1127-100, ATGP1127-250, ATGP1127-500, ATGP1127-1

References & Citations

Lv D., et al. (2011) IuBMB Life. 63 (1) :62-9. ; ; Chen BP., et al. (1994) J Biol Chem 269 (22) : 15819-26.

Other References

Sato A, et al. Role of activating transcription factor 3 protein ATF3 in necrosis and apoptosis induced by 5-fluoro-2-deoxyuridine. (FEBS J. 2014) {https://pubmed.ncbi.nlm.nih.gov/24529083/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001665

Uniprot Accession Number

P18847

Species

Human

AA Sequence

MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK LESVNAELKA QIEELKNEKQ HLIYMLNLHR PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ S