Recombinant human RAB13 protein

RAB13 (Ras-related protein Rab-13) belongs to the small GTPase superfamily. Members of the RAB protein family are nontransforming monomeric GTP-binding proteins of the Ras superfamily that contain 4 highly conserved regions involved in GTP binding and hydrolysis. RAB proteins are also an integral part of endocytic pathways. The GTPase Rab13 regulates the assembly of functional epithelial tight junctions (TJs) . It is expressed at all stages of preimplantation development. Recombinant human RAB13 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras-related protein Rab-13, GIG4, RAB13 member RAS oncogene family, Cell growth-inhibiting gene 4 protein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

24.7 kDa (220aa) confirmed by MALDI-TOF

Additionnal Information

Rab13, Ras-related protein Rab-13, GIG4, ATGP1126-10 µg, ATGP1126-20 µg, ATGP1126-50 µg, ATGP1126-100 µg, ATGP1126-250 µg, ATGP1126-500 µg, ATGP1126-1 mg, ATGP1126-10, ATGP1126-20, ATGP1126-50, ATGP1126-100, ATGP1126-250, ATGP1126-500, ATGP1126-1

References & Citations

Sheth B., et al. (2000) Mech Dev. 97 (1-2) :93-104.; ; Kohler K., et al. (2004) J Cell Biol. 165 (2) :175-80.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 200 mM NaCl, 1mM EDTA

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002861.1

Uniprot Accession Number

P51153

Species

Human

AA Sequence

MAKAYDHLFK LLLIGDSGVG KTCLIIRFAE DNFNNTYIST IGIDFKIRTV DIEGKKIKLQ VWDTAGQERF KTITTAYYRG AMGIILVYDI TDEKSFENIQ NWMKSIKENA SAGVERLLLG NKCDMEAKRK VQKEQADKLA REHGIRFFET SAKSSMNVDE AFSSLARDIL LKSGGRRSGN GNKPPSTDLK TCDKKNTNKC