Recombinant human Nanog protein
NANOG, also known as nanog homeobox, is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is down-regulated, cell differentiation can proceed. Recombinant human NANOG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Nanog homeobox, hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
19.6 kDa (174aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Clark A T., et al. (2004) Stem Cells. 22:169-179.; ; Chambers I., et al. (2003) Cell Res. 13:499-502.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_079141
Uniprot Accession Number
Q9H9S0
Species
Human
AA Sequence
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