Recombinant human Oncostatin M/OSM protein

OSM, also known as Oncostatin M, is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. Of these cytokines it most closely resembles leukemia inhibitory factor (LIF) in both structure and function. However, it is as yet poorly defined and is proving important in liver development, haematopoeisis, inflammation and possibly CNS development. It is also associated with bone formation and destruction. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR. Recombinant human OSM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Oncostatin M, MGC20461, Oncostatin M precursor

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

25.9 kDa (230aa) confirmed by MALDI-TOF

Additionnal Information

Oncostatin M, OSM, Oncostatin M, MGC20461, Oncostatin M precursor, ATGP1123-10 µg, ATGP1123-20 µg, ATGP1123-50 µg, ATGP1123-100 µg, ATGP1123-250 µg, ATGP1123-500 µg, ATGP1123-1 mg, ATGP1123-10, ATGP1123-20, ATGP1123-50, ATGP1123-100, ATGP1123-250, ATGP1123-500, ATGP1123-1

References & Citations

Nair B.C., et al. (1992) Science. 255:1430-1432; ; Miles S.A., et al. (1992) Science. 255:1432-1434

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_065391

Uniprot Accession Number

P13725

Species

Human

AA Sequence

AAIGSCSKE YRVLLGQLQK QTDLMQDTSR LLDPYIRIQG LDVPKLREHC RERPGAFPSE ETLRGLGRRG FLQTLNATLG CVLHRLADLE QRLPKAQDLE RSGLNIEDLE KLQMARPNIL GLRNNIYCMA QLLDNSDTAE PTKAGRGASQ PPTPTPASDA FQRKLEGCRF LHGYHRFMHS VGRVFSKWGE SPNRSRRHSP HQALRKGVRR