Recombinant human POU2AF1 protein

POU2AF1, also known as BOB1, is a lymphocyte specific transcription coactivator protein. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, POU2AF1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. POU2AF1 is expressed at highest levels in spleen and peripheral blood leukocytes. Recombinant human POU2AF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

POu domain class 2-associating factor 1, BOB1, OBF-1, OBF1, OCAB

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

29.6 kDa (276aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

POU2AF1, POu domain class 2-associating factor 1, BOB1, OBF-1, OBF1, OCAB, ATGP1122-10 µg, ATGP1122-20 µg, ATGP1122-50 µg, ATGP1122-100 µg, ATGP1122-250 µg, ATGP1122-500 µg, ATGP1122-1 mg, ATGP1122-10, ATGP1122-20, ATGP1122-50, ATGP1122-100, ATGP1122-250, ATGP1122-500, ATGP1122-1

References & Citations

Gstaiger M., et al. (1995) Nature. 373:360-362; ; Luo Y., et al. (1995) Mol. Cell. Biol. 15:4115-4124

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_006226

Uniprot Accession Number

Q16633

Species

Human

AA Sequence

MLWQKPTAPE QAPAPARPYQ GVRVKEPVKE LLRRKRGHAS SGAAPAPTAV VLPHQPLATY TTVGPSCLDM EGSVSAVTEE AALCAGWLSQ PTPATLQPLA PWTPYTEYVP HEAVSCPYSA DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTTRSSATPA VGPPLEGPEH QAPLTYFPWP QPLSTLPTST LQYQPPAPAL PGPQFVQLPI SIPEPVLQDM EDPRRAASSL TIDKLLLEEE DSDAYALNHT LSVEGF