Recombinant human SEPT5/SEPTIN5 protein

SEPT5, also known as septin-5, belongs to the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Recombinant human SEPT5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Septin 5, Peanut-like proein 1, PNUTL1, HCDCREL-1, H5, Cell division control-related protein 1, CDCrel-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

45.2 kDa (392aa) confirmed by MALDI-TOF

Additionnal Information

Septin-5, Septin 5, Peanut-like proein 1, PNUTL1, HCDCREL-1, H5, Cell division control-related protein 1, SEPT 5, SEPT-5, CDCREL, CDCREL-1, CDCREL1, ATGP1121-10 µg, ATGP1121-20 µg, ATGP1121-50 µg, ATGP1121-100 µg, ATGP1121-250 µg, ATGP1121-500 µg, ATGP1121-1 mg, ATGP1121-010, ATGP1121-020, ATGP1121-050, ATGP1121-100, ATGP1121-250, ATGP1121-500, ATGP1121-01M

References & Citations

Blaeser S., et al. (2002) FEBS Lett. 519:169-172; ; Hall P.A., et al. (2005) J. Pathol. 206:269-278

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM MES buffer (pH 5.0) containing 10% glycerol, 0.5M NaCl, 250 mM Imidazole, 0.1mM PMSF

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002679.2

Uniprot Accession Number

Q99719

Species

Human

AA Sequence

MSTGLRY KSKLATPEDK QDIDKQYVGF ATLPNQVHRK SVKKGFDFTL MVAGESGLGK STLVHSLFLT DLYKDRKLLS AEERISQTVE ILKHTVDIEE KGVKLKLTIV DTPGFGDAVN NTECWKPITD YVDQQFEQYF RDESGLNRKN IQDNRVHCCL YFISPFGHGL RPVDVGFMKA LHEKVNIVPL IAKADCLVPS EIRKLKERIR EEIDKFGIHV YQFPECDSDE DEDFKQQDRE LKESAPFAVI GSNTVVEAKG QRVRGRLYPW GIVEVENQAH CDFVKLRNML IRTHMHDLKD VTCDVHYENY RAHCIQQMTS KLTQDSRMES PIPILPLPTP DAETEKLIRM KDEELRRMQE MLQRMKQQMQ DQ