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Recombinant mouse S100A9 protein

S100A9, also known as Calgranulin-B, belongs to the S100 family containing EF-hand type Ca2+-binding proteins. It is involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation, and associated with the disease cystic fibrosis. S100A9 has been implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. This protein may function in the inhibition of casein kinase. Recombinant mouse S100A9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

S100 calcium binding protein A9, Calgranulin-B, Cagb, Mrp14

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

15.2 kDa (133aa) confirmed by MALDI-TOF

Additionnal Information

S100A9, S100 calcium binding protein A9, Calgranulin-B, Cagb, Mrp14, ATGP1120-10 µg, ATGP1120-20 µg, ATGP1120-50 µg, ATGP1120-100 µg, ATGP1120-250 µg, ATGP1120-500 µg, ATGP1120-1 mg, ATGP1120-10, ATGP1120-20, ATGP1120-50, ATGP1120-100, ATGP1120-250, ATGP1120-500, ATGP1120-1

References & Citations

Schafer BW., et al. (1996) Trends biochem Sci. 21 (4) :134-140; ; Nacken W., et al. (2003) Microsc Res Tech. 60:569-580

Other References

Takagi R, et al. S100A9 Increases IL-6 and RANKL Expressions through MAPKs and STAT3 Signaling Pathways in Osteocyte-Like Cells. (Biomed Res Int. 2020) {https://pubmed.ncbi.nlm.nih.gov/32149126/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_033140.1

Uniprot Accession Number

P31725

Species

Mouse

AA Sequence

MANKAPSQME RSITTIIDTF HQYSRKEGHP DTLSKKEFRQ MVEAQLATFM KKEKRNEALI NDIMEDLDTN QDNQLSFEEC MMLMAKLIFA CHEKLHENNP RGHGHSHGKG CGK

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