Recombinant human GLYAT protein

GLYAT, also known as glycine-N-acyltransferase, is a mitochondrial acyltransferase which conjugates glycine with acyl-CoA substrates in the mitochondria. The protein is thought to be important in the detoxification of endogenous and xenobiotic acyl-CoA's. Recombinant human GLYAT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Glycine-N-acyltransferase, ACGNAT, CAT, GAT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.0 kDa (316aa) confirmed by MALDI-TOF

Additionnal Information

GLYAT, Glycine-N-acyltransferase, ACGNAT, CAT, GAT, ATGP1119-10 µg, ATGP1119-20 µg, ATGP1119-50 µg, ATGP1119-100 µg, ATGP1119-250 µg, ATGP1119-500 µg, ATGP1119-1 mg, ATGP1119-10, ATGP1119-20, ATGP1119-50, ATGP1119-100, ATGP1119-250, ATGP1119-500, ATGP1119-1

References & Citations

Mawal Y.R., et al. (1994) Biochem. Biophys. Res. Commun. 205:1373-1379; ; Westhuizen F.H., et al. (2000) J. Biochem. Mol. Toxicol. 14:102-109

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_964011

Uniprot Accession Number

Q6IB77

Species

Human

AA Sequence

MMLPLQGAQM LQMLEKSLRK SLPASLKVYG TVFHINHGNP FNLKAVVDKW PDFNTVVVCP QEQDMTDDLD HYTNTYQIYS KDPQNCQEFL GSPELINWKQ HLQIQSSQPS LNEAIQNLAA IKSFKVKQTQ RILYMAAETA KELTPFLLKS KILSPSGGKP KAINQEMFKL SSMDVTHAHL VNKFWHFGGN ERSQRFIERC IQTFPTCCLL GPEGTPVCWD LMDQTGEMRM AGTLPEYRLH GLVTYVIYSH AQKLGKLGFP VYSHVDYSNE AMQKMSYTLQ HVPIPRSWNQ WNCVPL