Recombinant human Adenosine Deaminase/ADA protein
ADA, also known as adenosine deaminase, catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. This protein plays an important role in purine metabolism and in adenosine homeostasis. ADA acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Recombinant human ADA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Specifications
Specific activity is >40 units/mg, and is defined as the amount of enzyme that convert 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C.
Product Name Alternative
Adenosine deaminase, Adenosine aminohydrolase, ADA1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is >40unit/mg, and is defined as the amount of enzyme that convert 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C.
Molecular Weight
42.9 kDa (383aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gines S., et al. (2002) Biochem. 361:203-209; ; Daddona P.E., et al. (1984) J. Biol. Chem. 259:12101-12106
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_000013.2
Uniprot Accession Number
P00813
Species
Human
AA Sequence
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