Recombinant human Adenosine Deaminase/ADA protein

ADA, also known as adenosine deaminase, catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. This protein plays an important role in purine metabolism and in adenosine homeostasis. ADA acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Recombinant human ADA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is >40 units/mg, and is defined as the amount of enzyme that convert 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C.

Product Name Alternative

Adenosine deaminase, Adenosine aminohydrolase, ADA1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is >40unit/mg, and is defined as the amount of enzyme that convert 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C.

Molecular Weight

42.9 kDa (383aa) confirmed by MALDI-TOF

Additionnal Information

Adenosine Deaminase, ADA, Adenosine deaminase, Adenosine aminohydrolase, ADA1, ATGP1116-10 µg, ATGP1116-20 µg, ATGP1116-50 µg, ATGP1116-100 µg, ATGP1116-250 µg, ATGP1116-500 µg, ATGP1116-1 mg, ATGP1116-10, ATGP1116-20, ATGP1116-50, ATGP1116-100, ATGP1116-250, ATGP1116-500, ATGP1116-1

References & Citations

Gines S., et al. (2002) Biochem. 361:203-209; ; Daddona P.E., et al. (1984) J. Biol. Chem. 259:12101-12106

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000013.2

Uniprot Accession Number

P00813

Species

Human

AA Sequence

MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL