Recombinant human DTYMK protein

DTYMK is also known as thymidylate kinase family and is involved in pyrimidine metabolism. Specifically, DTYMK catalyzes the ATP-dependent conversion of dTMP (deoxythymidine monophosphate) to dTDP (deoxythymidine diphosphate), which then functions as one of the four nucleotides in DNA. Via its role in the catalytic creation of dTDP, DTYMK plays an important role in the pathway of DNA synthesis and is thought to be involved in cell cycle progression and cell growth. Recombinant human DTYMK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Thymidylate kinase, CDC8, FLJ44192, MGC198617, PP3731, TMPK, TYMK

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

26 kDa (232aa) confirmed by MALDI-TOF

Additionnal Information

DTYMK, Thymidylate kinase, CDC8, FLJ44192, MGC198617, PP3731, TMPK, TYMK, ATGP1114-10 µg, ATGP1114-20 µg, ATGP1114-50 µg, ATGP1114-100 µg, ATGP1114-250 µg, ATGP1114-500 µg, ATGP1114-1 mg, ATGP1114-10, ATGP1114-20, ATGP1114-50, ATGP1114-100, ATGP1114-250, ATGP1114-500, ATGP1114-1

References & Citations

Cummins RR., et al. (1980) Cancer Res. 40 (4) :1235-9.; ; Su JY., et al. (1991) Nucleic Acids Res. 19 (4) :823-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 0.1M NaCl

Scientific Category

Cell Cycle

NCBI Accession Number

NP_036277.2

Uniprot Accession Number

P23919

Species

Human

AA Sequence

MAARRGALIV LEGVDRAGKS TQSRKLVEAL CAAGHRAELL RFPERSTEIG KLLSSYLQKK SDVEDHSVHL LFSANRWEQV PLIKEKLSQG VTLVVDRYAF SGVAFTGAKE NFSLDWCKQP DVGLPKPDLV LFLQLQLADA AKRGAFGHER YENGAFQERA LRCFHQLMKD TTLNWKMVDA SKSIEAVHED IRVLSEDAIR TATEKPLGEL WK