Recombinant human NEDP1/SENP8 protein

SENP8 is a cysteine protease that is a member of the sentrin-specific protease family. This protein is involved in processing and deconjugation of the ubiquitin-like protein termed, neural precursor cell expressed developmentally downregulated 8 (NEDD8) . Recombinant human SENP8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

SuMO/sentrin specific peptidase family member 8, DEN1, NEDP1, PRSC2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

26.2 kDa (232aa) confirmed by MALDI-TOF

Additionnal Information

NEDP1, SENP8, SuMO/sentrin specific peptidase family member 8, DEN1, NEDP1, PRSC2, ATGP1112-10 µg, ATGP1112-20 µg, ATGP1112-50 µg, ATGP1112-100 µg, ATGP1112-250 µg, ATGP1112-500 µg, ATGP1112-1 mg, ATGP1112-10, ATGP1112-20, ATGP1112-50, ATGP1112-100, ATGP1112-250, ATGP1112-500, ATGP1112-1

References & Citations

Reverter D., et al. (2004) Atructure. 12 (8) :1519-31.; ; Shen LN., et al. (2005) EMBO J. 24 (7) :1341-51.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Scientific Category

Ubiquitination

NCBI Accession Number

NP_001159812

Uniprot Accession Number

Q96LD8

Species

Human

AA Sequence

MDPVVLSYMD SLLRQSDVSL LDPPSWLNDH IIGFAFEYFA NSQFHDCSDH VSFISPEVTQ FIKCTSNPAE IAMFLEPLDL PNKRVVFLAI NDNSNQAAGG THWSLLVYLQ DKNSFFHYDS HSRSNSVHAK QVAEKLEAFL GRKGDKLAFV EEKAPAQQNS YDCGMYVICN TEALCQNFFR QQTESLLQLL TPAYITKKRG EWKDLITTLA KK