Recombinant human NAT1 protein

NAT1 belongs to the arylamine N-acetyltransferase family. It catalyzes N- or O-acetylation of heterocyclic and arylamine substrates in the detoxification of a wide array of drugs. Certain alleles causing high levels of N-acetyltransferase activity have been associated with colon and urinary bladder cancers, as NAT1 also bioactivate several known carcinogens. This enzyme helps metabolize drugs and other xenobiotics, and participates in the detoxification of a plethora of hydrazine and arylamine drugs. Recombinant human NAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Arylamine N-acetyltransferase 1, AAC1, MNAT, NAT-1, NATI

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

36.1 kDa (310aa) confirmed by MALDI-TOF

Additionnal Information

NAT1, Arylamine N-acetyltransferase 1, AAC1, MNAT, NAT-1, NATI, ATGP1110-10 µg, ATGP1110-20 µg, ATGP1110-50 µg, ATGP1110-100 µg, ATGP1110-250 µg, ATGP1110-500 µg, ATGP1110-1 mg, ATGP1110-10, ATGP1110-20, ATGP1110-50, ATGP1110-100, ATGP1110-250, ATGP1110-500, ATGP1110-1

References & Citations

Gu J., et al. (2005) Multat Res. 581 (1-2) :97-104.; ; Kiss I., et al. (2004) Anticancer Res. 24 (6) :3965-70.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Scientific Category

Metabolism

NCBI Accession Number

NP_001153644.1

Uniprot Accession Number

P18440

Species

Human

AA Sequence

MDIEAYLERI GYKKSRNKLD LETLTDILQH QIRAVPFENL NIHCGDAMDL GLEAIFDQVV RRNRGGWCLQ VNHLLYWALT TIGFETTMLG GYVYSTPAKK YSTGMIHLLL QVTIDGRNYI VDAGFGRSYQ MWQPLELISG KDQPQVPCIF RLTEENGFWY LDQIRREQYI PNEEFLHSDL LEDSKYRKIY SFTLKPRTIE DFESMNTYLQ TSPASVFTSK SFCSLQTPDG VHCLVGFTLT HRRFNYKDNT DLIEFKTLSE EEIEKVLKNI FNISLQRKLV PKHGDRFFTI