Recombinant human VPS24/CHMP3 protein
VPS24, also known as Charged multivesicular body protein 3 (CHMP3), belongs to the vacuolar sorting protein family and function as chromatin modifying proteins. It associates directly with CHMP2 and CHMP4 for the disassembly of ESCRT-III complex in an ATP-dependent manner. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins. Overexpression of VPS24 strongly inhibits HIV-1 release. Recombinant human VPS24 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Charged multivesicular body protein 3, CGI-149, CHMP3, FLJ38114, NEDF
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
27.2 kDa (242aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Muziol T. et al. (2006) Dev. Cell. 10: 821-830.; ; Bache K.G. et al. (2006) Mol. Biol. Cell 17: 2513-2523.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT
Scientific Category
Cell Cycle
NCBI Accession Number
NP_057163
Uniprot Accession Number
Q9Y3E7
Species
Human
AA Sequence
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