Recombinant human RAB3D protein

Ras-related protein Rab-3D, also known as GOV, is a member of the Ras superfamily of small Rab GTPases. Rab proteins play an important role for, either in endocytosis or in biosynthetic protein transport. Rab3D mRNA is predominantly expressed in white adipose tissue and, coincident with GLuT4, increases upon differentiation of 3T3-L1 broblasts into insulin-responsive adipocytes. Rab3D displays close structural similarities with Rab 3A, an isoform which has been functionally implicated in regulated exocytosis in various cell types. Recombinant human Rab3D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

RAB3D member RAS oncogene family, Ras-related protein Rab-3D, Rab3D upregulated with myeloid differentiation, glioblastoma overexpressed GOV, RAB16, D2-2, RAD3D

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.4 kDa (239aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

RAB3D, Ras-related protein Rab-3D, GOV, RAB16, ATGP1102-10 µg, ATGP1102-20 µg, ATGP1102-50 µg, ATGP1102-100 µg, ATGP1102-250 µg, ATGP1102-500 µg, ATGP1102-1 mg, ATGP1102-10, ATGP1102-20, ATGP1102-50, ATGP1102-100, ATGP1102-250, ATGP1102-500, ATGP1102-1

References & Citations

Zahraoui A., et al. (1989) J Biol Chem. 264:12934-12401.; ; Baldini G., et al. (1992) Proc Natl Acad Sci. 89:5049-5052.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.2M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004274

Uniprot Accession Number

O95716

Species

Human

AA Sequence

MASAGDTQAG PRDAADQNFD YMFKLLLIGN SSVGKTSFLF RYADDSFTPA FVSTVGIDFK VKTVYRHDKR IKLQIWDTAG QERYRTITTA YYRGAMGFLL MYDIANQESF AAVQDWATQI KTYSWDNAQV ILVGNKCDLE DERVVPAEDG RRLADDLGFE FFEASAKENI NVKQVFERLV DVICEKMNES LEPSSSSGSN GKGPAVGDAP APQPSSCSC