Recombinant human ARF5 protein
ADP-ribosylation factor 5, also known as ARF5, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of actin to the golgi via rho family-dependent G-protein localization and WASP/Arp2/3 complexes. ARF5 is involved in vesicular transport and functioning via phospholipase D activation. Recombinant human ARF5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
ADP-ribosylation factor 5, ADP ribosylation factor 5, ARF 5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
22.6 kDa (200aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Randazzo P A., et al. (1994) J Biol Chem. 269:29490-29494.; ; Fucini R V., et al. (2000) J Biol Chem. 275:18824-18829.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001653
Uniprot Accession Number
P84085
Species
Human
AA Sequence
Frequently Asked Questions
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