Recombinant human ARF5 protein

ADP-ribosylation factor 5, also known as ARF5, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of actin to the golgi via rho family-dependent G-protein localization and WASP/Arp2/3 complexes. ARF5 is involved in vesicular transport and functioning via phospholipase D activation. Recombinant human ARF5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

ADP-ribosylation factor 5, ADP ribosylation factor 5, ARF 5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

22.6 kDa (200aa) confirmed by MALDI-TOF

Additionnal Information

ARF5, ADP-ribosylation factor 5, ADP ribosylation factor 5, ARF 5, ATGP1100-10 µg, ATGP1100-20 µg, ATGP1100-50 µg, ATGP1100-100 µg, ATGP1100-250 µg, ATGP1100-500 µg, ATGP1100-1 mg, ATGP1100-10, ATGP1100-20, ATGP1100-50, ATGP1100-100, ATGP1100-250, ATGP1100-500, ATGP1100-1

References & Citations

Randazzo P A., et al. (1994) J Biol Chem. 269:29490-29494.; ; Fucini R V., et al. (2000) J Biol Chem. 275:18824-18829.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001653

Uniprot Accession Number

P84085

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGLTVSALFS RIFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV QESADELQKM LQEDELRDAV LLVFANKQDM PNAMPVSELT DKLGLQHLRS RTWYVQATCA TQGTGLYDGL DWLSHELSKR