Recombinant human RAB2A protein

RAB2A (Ras-related protein Rab-2A) belongs to the small GTPase superfamily. Members of the RAB protein family are nontransforming monomeric GTP-binding proteins of the Ras superfamily that contain 4 highly conserved regions involved in GTP binding and hydrolysis. RAB proteins are also an integral part of endocytic pathways. RAB2A and RAB2B are required for protein transport from the ER to the Golgi, RAB2A is lipid-anchored to the ER-Golgi intermediate compartment membrane while RAB2B is lipid anchored to the cytoplasmic side of the cell membrane. RAB2A has been shown to interact with GOLGA2. Recombinant human RAB2A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras-related protein Rab-2A, RAB2, RAB2A member RAS oncogene family

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.1 kDa (236aa) confirmed by MALDI-TOF

Additionnal Information

RAB2A, ras-related protein Rab-2A, RAB2, ATGP1098-10 µg, ATGP1098-20 µg, ATGP1098-50 µg, ATGP1098-100 µg, ATGP1098-250 µg, ATGP1098-500 µg, ATGP1098-1 mg, ATGP1098-10, ATGP1098-20, ATGP1098-50, ATGP1098-100, ATGP1098-250, ATGP1098-500, ATGP1098-1

References & Citations

Opdam, F.J., et al. (2000) Eur. J. Cell Biol. 79: 308-316.; ; Tisdale E.J. (2003) J. Biol. Chem. 278:52524-52530

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002856.1

Uniprot Accession Number

P61019

Species

Human

AA Sequence

MAYAYL FKYIIIGDTG VGKSCLLLQF TDKRFQPVHD LTIGVEFGAR MITIDGKQIK LQIWDTAGQE SFRSITRSYY RGAAGALLVY DITRRDTFNH LTTWLEDARQ HSNSNMVIML IGNKSDLESR REVKKEEGEA FAREHGLIFM ETSAKTASNV EEAFINTAKE IYEKIQEGVF DINNEANGIK IGPQHAATNA THAGNQGGQQ AGGGCC