Recombinant human CDC26 protein
CDC26, also known as ANAPC12, APC12, belongs to the CDC26 family which orchestrates progression through mitosis by decorating cell-cycle regulators with ubiquitin chains. This protein is component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins. Recombinant human CDC26 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Anaphase-promoting complex subunit CDC26, ANAPC12, APC12
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
>85% by SDS-PAGE
Molecular Weight
11.9 kDa (105aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Jin L., et al. (2008) Cell. 133:653-665; ; Gmachl M., et al. (2000) Proc. Natl. Acad. Sci. u.S.A. 97:8973-8978
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl
Scientific Category
Cell Cycle
NCBI Accession Number
NP_644815
Uniprot Accession Number
Q8NHZ8
Species
Human
AA Sequence
MLRRKPTRLE LKLDDIEEFE NIRKDLETRK KQKEDVEVVG GSDGEGAIGL SSDPKSREQM INDRIGYKPQ PKPNNRSSQF GSLEF
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