Recombinant human TRANCE/RANK L/TNFSF11 protein

TNFSF11, also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. TNFSF11 also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. TNFSF11 is important in bone metabolism. This natural and necessary surface-bound molecule (also known as CD254) found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption. Recombinant human TNFSF11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

22.3 kDa (199aa) confirmed by MALDI-TOF

Additionnal Information

TRANCE , TNFSF11, RANK L, Tumor necrosis factor ligand superfamily member 11, OPGL, RANKL, ATGP1093-10 µg, ATGP1093-20 µg, ATGP1093-50 µg, ATGP1093-100 µg, ATGP1093-250 µg, ATGP1093-500 µg, ATGP1093-1 mg, ATGP1093-10, ATGP1093-20, ATGP1093-50, ATGP1093-100, ATGP1093-250, ATGP1093-500, ATGP1093-1

References & Citations

Lam J., et al. (2001) J. Clin. Invest. 108:971-979; ; Ito S., et al. (2002) J. Biol. Chem. 277:6631-6636

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_003692

Uniprot Accession Number

O14788

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MIRAEKAMVD GSWLDLAKRS KLEAQPFAHL TINATDIPSG SHKVSLSSWY HDRGWAKISN MTFSNGKLIV NQDGFYYLYA NICFRHHETS GDLATEYLQL MVYVTKTSIK IPSSHTLMKG GSTKYWSGNS EFHFYSINVG GFFKLRSGEE ISIEVSNPSL LDPDQDATYF GAFKVRDID