Recombinant human Synaptotagmin-1 protein

Synaptotagmin-1 (SYT1) belongs to the synaptotagmin family contains 2 C2 domains. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis. SYT1 is the master switch responsible for allowing the human brain to release neurotransmitters. Recombinant human SYT1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Synaptotagmin 1, P65, SVP65, SYT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

29.5 kDa (256aa) confirmed by MALDI-TOF

Additionnal Information

Synaptotagmin1, synaptotagmin 1, P65, SVP65, SYT, ATGP1091-10 µg, ATGP1091-20 µg, ATGP1091-50 µg, ATGP1091-100 µg, ATGP1091-250 µg, ATGP1091-500 µg, ATGP1091-1 mg, ATGP1091-10, ATGP1091-20, ATGP1091-50, ATGP1091-100, ATGP1091-250, ATGP1091-500, ATGP1091-1

References & Citations

Han C., et al. (2009) J. Immunol. 182:2986-2996; ; Xi, D., et al. (1999) Neuroscience 88: 425-435.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100 mM NaCl

Scientific Category

Neuroscience

NCBI Accession Number

NP_005630

Uniprot Accession Number

P21579

Species

Human

AA Sequence

MEPKEEEKLG KLQYSLDYDF QNNQLLVGII QAAELPALDM GGTSDPYVKV FLLPDKKKKF ETKVHRKTLN PVFNEQFTFK VPYSELGGKT LVMAVYDFDR FSKHDIIGEF KVPMNTVDFG HVTEEWRDLQ SAEKEEQEKL GDICFSLRYV PTAGKLTVVI LEAKNLKKMD VGGLSDPYVK IHLMQNGKRL KKKKTTIKKN TLNPYYNESF SFEVPFEQIQ KVQVVVTVLD YDKIGKNDAI GKVFVGYNLE HHHHHH