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Recombinant mouse S100A1 protein

S100 calcium binding protein A1, also known as S100A, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A1 is expressed by striated muscle, endothelium and chondrocytes. Intracellularly, S100A1 regulates cytosolic Ca levels, and is part of the Hsp70/Hsp90 mutlichaperone complex that protects against protein denaturation. Recombinant mouse S100A1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

S100 calcium binding protein A1, S100, S100a

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

12.6 kDa (114aa) confirmed by MALDI-TOF

Additionnal Information

S100A1, S100 calcium binding protein A1, S100, S100a, ATGP1089-10 µg, ATGP1089-20 µg, ATGP1089-50 µg, ATGP1089-100 µg, ATGP1089-250 µg, ATGP1089-500 µg, ATGP1089-1 mg, ATGP1089-10, ATGP1089-20, ATGP1089-50, ATGP1089-100, ATGP1089-250, ATGP1089-500, ATGP1089-1

References & Citations

Heizmann C W., et al. (1988) J Cardiovasc Pharmacol. 5:30-37.; ; Kagi u., et al. (1988) Cell Tissue Res. 252:359-365.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_035439

Uniprot Accession Number

P56565

Species

Mouse

AA Sequence

MGSELESAME TLINVFHAHS GKEGDKYKLS KKELKDLLQT ELSGFLDVQK DADAVDKVMK ELDENGDGEV DFKEYVVLVA ALTVACNNFF WETS

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