Recombinant human AP1S2 protein

AP1S2, also known as AP-1 complex subunit sigma-2, serves as the small subunit of AP-1 complex 1 and is a member of the adaptin protein family. Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. Recombinant human AP1S2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

AP-1 complex subunit sigma-2, DC22, MGC:1902, MRX59, SIGMA1B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

20.7 kDa (177aa) confirmed by MALDI-TOF

Additionnal Information

AP1S2, AP-1 complex subunit sigma-2, DC22, MGC:1902, MRX59, SIGMA1B, ATGP1086-10 µg, ATGP1086-20 µg, ATGP1086-50 µg, ATGP1086-100 µg, ATGP1086-250 µg, ATGP1086-500 µg, ATGP1086-1 mg, ATGP1086-10, ATGP1086-20, ATGP1086-50, ATGP1086-100, ATGP1086-250, ATGP1086-500, ATGP1086-1

References & Citations

Tarpey PS. et al. (2006) Am J Hum Genet. 79:1119-24.; ; Takatsu H. et al. (1998) J Biol Chem. 273 :24693-700

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl,2mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003907

Uniprot Accession Number

P56377

Species

Human

AA Sequence

MQFMLLFSRQ GKLRLQKWYV PLSDKEKKKI TRELVQTVLA RKPKMCSFLE WRDLKIVYKR YASLYFCCAI EDQDNELITL EIIHRYVELL DKYFGSVCEL DIIFNFEKAY FILDEFLLGG EVQETSKKNV LKAIEQADLL QEEAETPRSV LEEIGLT