Recombinant human UBL5 protein
UBL5, also known ubiquitin-like protein 5, is thought to be reversible modulators of protein function rather than protein degraders like ubiquitin. This protein effects a nuclear step required for mounting a response to the threat of mitochondrial protein misfolding. Recombinant human UBL5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Ubiquitin-like protein 5, 1110030M22Rik, beacon
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
10.7 kDa (93aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Friedman JS. et al. (2001) Genomics 71:252-5.; ; McNally T. et al. (2003) Protein Sci. 12:1562-6.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,2mM DTT
Scientific Category
Ubiquitination
NCBI Accession Number
NP_077268
Uniprot Accession Number
Q9BZL1
Species
Human
AA Sequence
MIEVVCNDRL GKKVRVKCNT DDTIGDLKKL IAAQTGTRWN KIVLKKWYTI FKDHVSLGDY EIHDGMNLEL YYQ
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