Recombinant human UBL3 protein
UBL3, also known as PNSC1, is a 117 amino acid membrane protein belonging to the ubiquitin-like family. This protein contains two potential N-glycosylation sites, a potential protein kinase C phosphorylation site and a potential C-terminal prenylation site. ubiquitin-like proteins are not directly involved in protein degradation, but appear to have many mechanistic similarities with the ubiquitin pathway. Recombinant human uBL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ubiquitin-like protein 3, DKFZp434K151, FLJ32018, HCG-1, PNSC1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Molecular Weight
15 kDa (134aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Downes BP., et al. (2006) J Biol Chem. 281 (37) :27145-57.; ; Leung A., et al. (2008) Ernst Schering Found Symp Proc. (1) :75-97.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 0.1M NaCl.
Scientific Category
Ubiquitination
NCBI Accession Number
NP_009037
Uniprot Accession Number
O95164
Species
Human
AA Sequence
MSSNVPADMI NLRLILVSGK TKEFLFSPND SASDIAKHVY DNWPMDWEEE QVSSPNILRL IYQGRFLHGN VTLGALKLPF GKTTVMHLVA RETLPEPNSQ GQRNREKTGE SNCC
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