Recombinant human Ubc16/UBE2W protein

UBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. This protein in vitro catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination. uBE2W is widely expressed, especially at highest levels in testis. Recombinant human uBE2W protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin conjugating enzyme E2 W, E2 ubiquitin-conjugating enzyme W, N-terminal E2 ubiquitin-conjugating enzyme, N-terminus-conjugating E2, Ubiquitin carrier protein W, Ubiquitin-conjugating enzyme 16, UBC-16, Ubiquitin-protein ligase W

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.4 kDa (171aa) confirmed by MALDI-TOF

Additionnal Information

UBE2W, Probable ubiquitin-conjugating enzyme E2 W, FLJ11011, huBC-16, ATGP1079-10 µg, ATGP1079-20 µg, ATGP1079-50 µg, ATGP1079-100 µg, ATGP1079-250 µg, ATGP1079-500 µg, ATGP1079-1 mg, ATGP1079-10, ATGP1079-20, ATGP1079-50, ATGP1079-100, ATGP1079-250, ATGP1079-500, ATGP1079-1

References & Citations

David Y., et al. (2010) J Biol Chem. 285 (12) :8595-604

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Ubiquitination

NCBI Accession Number

NP_060769.5

Uniprot Accession Number

Q96B02

Species

Human

AA Sequence

MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C