Recombinant e.coli ansA protein

AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB) . AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E. coli ansA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cytoplasmic L-asparaginase I, ECK1765, JW1756

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

39.3 kDa (358aa) confirmed by MALDI-TOF

Additionnal Information

AnsA, Cytoplasmic L-asparaginase I, ECK1765, JW1756, ATGP1077-10 µg, ATGP1077-20 µg, ATGP1077-50 µg, ATGP1077-100 µg, ATGP1077-250 µg, ATGP1077-500 µg, ATGP1077-1 mg, ATGP1077-10, ATGP1077-20, ATGP1077-50, ATGP1077-100, ATGP1077-250, ATGP1077-500, ATGP1077-1

References & Citations

Cantor JR., et al. (2009) Biochemistry. 48 (46) :11026-31. ; ; Weidle uH., et al. (2009) Anticancer Res. 29 (4) :951-63.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_416281

Uniprot Accession Number

P0A962

Species

E.coli

AA Sequence

MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT IHEYTPLMDS SDMTPEDWQH IAEDIKAHYD DYDGFVILHG TDTMAYTASA LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA NYPINEVTLF FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG ELIVHPITPQ PIGVVTIYPG ISADVVRNFL RQPVKALILR SYGVGNAPQN KAFLQELQEA SDRGIVVVNL TQCMSGKVNM GGYATGNALA HAGVIGGADM TVEATLTKLH YLLSQELDTE TIRKAMSQNL RGELTPDD