Recombinant e.coli ansA protein
AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB) . AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E. coli ansA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cytoplasmic L-asparaginase I, ECK1765, JW1756
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
39.3 kDa (358aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Cantor JR., et al. (2009) Biochemistry. 48 (46) :11026-31. ; ; Weidle uH., et al. (2009) Anticancer Res. 29 (4) :951-63.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_416281
Uniprot Accession Number
P0A962
Species
E.coli
AA Sequence
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