Recombinant human ARPC5 protein
P16-ARC, also known as ARPC5, is a 151 amino acid subunit of the Arp2/3 complex. Thought to play a role in maintaining the integrity of Arp2/3, ARPC5 is a substrate for MAPKAPK-2 which, through phosphorylation of ARPC5, may participate in Arp2/3 regulatory functions and remodeling of the Actin cytoskeleton. Two isoforms of ARPC5 exist due to alternative splicing events. Recombinant human ARPC5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Actin related protein 2/3 complex subunit 5, Actin related protein 2/3 complex, subunit 5, p16-ARC, ARC16
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
18.4 kDa (171aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Machesky L M., et al. (1998) Biochem J. 328:105-112.; ; Welch M D., et al. (1997) J Cell Biol. 138:375-384.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.1M NaCl
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_005708
Uniprot Accession Number
O15511
Species
Human
AA Sequence
Frequently Asked Questions
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