Recombinant human ARPC5 protein

P16-ARC, also known as ARPC5, is a 151 amino acid subunit of the Arp2/3 complex. Thought to play a role in maintaining the integrity of Arp2/3, ARPC5 is a substrate for MAPKAPK-2 which, through phosphorylation of ARPC5, may participate in Arp2/3 regulatory functions and remodeling of the Actin cytoskeleton. Two isoforms of ARPC5 exist due to alternative splicing events. Recombinant human ARPC5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Actin related protein 2/3 complex subunit 5, Actin related protein 2/3 complex, subunit 5, p16-ARC, ARC16

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

18.4 kDa (171aa) confirmed by MALDI-TOF

Additionnal Information

ARPC5, Actin related protein 2/3 complex subunit 5, Actin related protein 2/3 complex, subunit 5, p16-ARC, ARC16, ATGP1075-10 µg, ATGP1075-20 µg, ATGP1075-50 µg, ATGP1075-100 µg, ATGP1075-250 µg, ATGP1075-500 µg, ATGP1075-1 mg, ATGP1075-10, ATGP1075-20, ATGP1075-50, ATGP1075-100, ATGP1075-250, ATGP1075-500, ATGP1075-1

References & Citations

Machesky L M., et al. (1998) Biochem J. 328:105-112.; ; Welch M D., et al. (1997) J Cell Biol. 138:375-384.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.1M NaCl

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_005708

Uniprot Accession Number

O15511

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSKNTVSSAR FRKVDVDEYD ENKFVDEEDG GDGQAGPDEG EVDSCLRQGN MTAALQAALK NPPINTKSQA VKDRAGSIVL KVLISFKAND IEKAVQSLDK NGVDLLMKYI YKGFESPSDN SSAMLLQWHE KALAAGGVGS IVRVLTARKT V