Recombinant human CLPP protein

CLPP (ATP-dependent Clp protease proteolytic subunit) belongs to the peptidase family S14. CLPP cleaves peptides in various proteins in a process that requires ATP hydrolysis. CLPP, the catalytic core of the Clp proteolytic complex, is widely involved in many cellular processes via the regulation of intracellular protein quality. CLPP may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. Recombinant human CLPP protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Putative ATP-dependent Clp protease proteolytic subunit mitochondrial, Endopeptidase Clp

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

24.2 kDa (222aa) confirmed by MALDI-TOF

Additionnal Information

CLPP, Putative ATP-dependent Clp protease proteolytic subunit mitochondrial, Endopeptidase Clp, ATGP1073-10 µg, ATGP1073-20 µg, ATGP1073-50 µg, ATGP1073-100 µg, ATGP1073-250 µg, ATGP1073-500 µg, ATGP1073-1 mg, ATGP1073-10, ATGP1073-20, ATGP1073-50, ATGP1073-100, ATGP1073-250, ATGP1073-500, ATGP1073-1

References & Citations

Li XH., et al (2010) BMC Microbiol. 19:10:54.; ; Kang, S.G., et al (2002) J. Biol. Chem. 277: 21095-21102.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 20% glycerol, 100 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_006003.1

Uniprot Accession Number

Q16740

Species

Human

AA Sequence

MPLIPIVVEQ TGRGERAYDI YSRLLRERIV CVMGPIDDSV ASLVIAQLLF LQSESNKKPI HMYINSPGGV VTAGLAIYDT MQYILNPICT WCVGQAASMG SLLLAAGTPG MRHSLPNSRI MIHQPSGGAR GQATDIAIQA EEIMKLKKQL YNIYAKHTKQ SLQVIESAME RDRYMSPMEA QEFGILDKVL VHPPQDGEDE PTLVQKEPVE AAPAAEPVPA ST