Recombinant human TSEN15 protein
TSEN15 (tRNA-splicing endonuclease subunit Sen15) belongs to the SEN15 family. tRNA splicing is a fundamental process required for cell growth and division. SEN15 is a subunit of the tRNA splicing endonuclease. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. Recombinant human TSEN15 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
TRNA-splicing endonuclease subunit Sen15, sen15, C1orf19
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
20.9 kDa (191aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Song J, et al. (2007) J Mol Biol 366 (1) : 155-64.; ; Paushkin, et al. (2004) Cell 117 (3) : 311-21.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_443197
Uniprot Accession Number
Q8WW01
Species
Human
AA Sequence
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