Recombinant human SNIP1 protein

SNIP1 is smad nuclear interacting protein contains a forkhead-associated (FHA) domain and acts as a nuclear inhibitor of CBP/p300. This protein is an inhibitor of the TGF-beta signal transduction pathway and to be important in suppressing transcriptional activation dependent on the co-activators CBP and p300. Inhibition of NF-kappa B activity is a function of the N-terminal domain of SNIP1 and involves competition of SNIP1 and the NF-kappa B subunit, RelA/p65, for binding to p300, similar to the mechanism of inhibition of Smad signaling by SNIP1. Recombinant human SNIP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Smad nuclear interacting protein 1, FLJ12553, Splicing factor arginine/serine rich 4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

18.7 kDa (160aa) confirmed by MALDI-TOF

Additionnal Information

SNIP1, Smad nuclear interacting protein 1, FLJ12553, Splicing factor arginine/serine rich 4, ATGP1071-10 µg, ATGP1071-20 µg, ATGP1071-50 µg, ATGP1071-100 µg, ATGP1071-250 µg, ATGP1071-500 µg, ATGP1071-1 mg, ATGP1071-10, ATGP1071-20, ATGP1071-50, ATGP1071-100, ATGP1071-250, ATGP1071-500, ATGP1071-1

References & Citations

Kim RH, et al. (2000) Genes Dev. 14 (13) :1605-16.; ; Kim RH, et al. (2001) J Biol Chem. 276 (49) :46297-304.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100 mM NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_078976

Uniprot Accession Number

Q8TAD8

Species

Human

AA Sequence

RWRLYPFKN DEVLPVMYIH RQSAYLLGRH RRIADIPIDH PSCSKQHAVF QYRLVEYTRA DGTVGRRVKP YIIDLGSGNG TFLNNKRIEP QRYYELKEKD VLKFGFSSRE YVLLHESSDT SEIDRKDDED EEEEEEVSDS