Recombinant human UCH-L5/UCH37 protein
Ubiquitin carboxyl-terminal hydrolase isozyme L5 (uCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Recombinant human uCHL5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity > 500 pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity: > 500pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.
Molecular Weight
39.7 kDa (349aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Yao T., et al. (2006) Nat. Cell Biol. 8:994-1002; ; Nishio K, et al. (2009) Biochem Biophys Res Commun. 390 (3) :855-60.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 200 mM NaCl, 0.1mM PMSF, 2mM EDTA
Scientific Category
Ubiquitination
NCBI Accession Number
NP_057068
Uniprot Accession Number
Q9Y5K5
Species
Human
AA Sequence
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