What is the catalog number for Recombinant human UCH-L5/UCH37 protein?

The catalog number for Recombinant human UCH-L5/UCH37 protein is ATGP1069-020.

What sizes are available for Recombinant human UCH-L5/UCH37 protein?

Recombinant human UCH-L5/UCH37 protein is available in the following size(s): 20 µg.

Is Recombinant human UCH-L5/UCH37 protein in stock?

Recombinant human UCH-L5/UCH37 protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human UCH-L5/UCH37 protein

Ubiquitin carboxyl-terminal hydrolase isozyme L5 (uCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Recombinant human uCHL5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity > 500 pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.

Product Name Alternative

Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin thioesterase L5, INO80 complex subunit R, INO80R, UCH37, CGI-70, AD-019

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity: > 500pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.

Molecular Weight

39.7 kDa (349aa) confirmed by MALDI-TOF

Additionnal Information

UCH-L5, UCHL5, UCH-37, UCH37, UCH L5, UCH 37, Ubiquitin thioesterase L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin carboxyl terminal hydrolase isozyme L5, Ubiquitin C terminal hydrolase UCH37, INO80R, INO80 complex subunit R, CGI-70, CGI70, CGI 70, AD-019, AD019, AD 019, ATGP1069-10 µg, ATGP1069-20 µg, ATGP1069-50 µg, ATGP1069-100 µg, ATGP1069-250 µg, ATGP1069-500 µg, ATGP1069-1 mg, ATGP1069-010, ATGP1069-020, ATGP1069-050, ATGP1069-100, ATGP1069-250, ATGP1069-500, ATGP1069-01M

References & Citations

Yao T., et al. (2006) Nat. Cell Biol. 8:994-1002; ; Nishio K, et al. (2009) Biochem Biophys Res Commun. 390 (3) :855-60.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 200 mM NaCl, 0.1mM PMSF, 2mM EDTA

Scientific Category

Ubiquitination

NCBI Accession Number

NP_057068

Uniprot Accession Number

Q9Y5K5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK