Recombinant human RNMT protein

RNMT (mRNA cap guanine-N7 methyltransferase), also known as MET, RG7MT1 or hCMT1c, is a widely expressed nuclear protein that belongs to the mRNA cap methyltransferase family. Cap-dependent mRNA translation requires the methylation of the mRNA guanosine cap by RNMT. RNMT catalyzes the transfer of a methyl group from AdoMet (S-adenosylmethionine) to the GpppN end of the growing mRNA at the N-7 position, thereby producing AdoHyc (S-adenosylhomocysteine) and m7GpppN terminated RNA. Recombinant human RNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

MRNA cap guanine-N7 methyltransferase, MET, RG7MT1, DKFZp686H1252, EC 2.1.1.56, hcm1p, hCMT1, HCMT1a, hCMT1c, hMet, hypothetical protein KIAA0396, KIAA0398, MET, mRNA (guanine 7) methyltransferase, mRNA (guanine N (7) ) methyltransferase, mRNA cap methyltransferase, OTTHuMP00000162509, RNA guanine 7 methyltransferase, RNA guanine-7-methyltransferase, RNMT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

57 kDa (496aa)

Additionnal Information

RNMT, mRNA cap guanine-N7 methyltransferase, MET, RG7MT1, DKFZp686H1252, EC 2.1.1.56, hcm1p, hCMT1, HCMT1a, hCMT1c, hMet, hypothetical protein KIAA0396, KIAA0398, MET, mRNA (guanine 7) methyltransferase, mRNA (guanine N (7) ) methyltransferase, mRNA cap methyltransferase, OTTHuMP00000162509, RNA guanine 7 methyltransferase, RNA guanine-7-methyltransferase, RNMT, ATGP1068-10 µg, ATGP1068-20 µg, ATGP1068-50 µg, ATGP1068-100 µg, ATGP1068-250 µg, ATGP1068-500 µg, ATGP1068-1 mg, ATGP1068-10, ATGP1068-20, ATGP1068-50, ATGP1068-100, ATGP1068-250, ATGP1068-500, ATGP1068-1

References & Citations

Cowling VH, et al. (2010) Genes Cancer. 1 (6) :576-579.; ; Cowling VH, et al. (2010) Oncogene. 29 (6) :930-6.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100 mM NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_003790.1

Uniprot Accession Number

O43148

Species

Human

AA Sequence

MANSAKAEEY EKMSLEQAKA SVNSETESSF NINENTTASG TGLSEKTSVC RQVDIARKRK EFEDDLVKES SSCGKDTPSK KRKLDPEIVP EEKDCGDAEG NSKKRKRETE DVPKDKSSTG DGTQNKRKIA LEDVPEKQKN LEEGHSSTVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK SVLIGEFLEK VRQKKKRDIT VLDLGCGKGG DLLKWKKGRI NKLVCTDIAD VSVKQCQQRY EDMKNRRDSE YIFSAEFITA DSSKELLIDK FRDPQMCFDI CSCQFVCHYS FESYEQADMM LRNACERLSP GGYFIGTTPN SFELIRRLEA SETESFGNEI YTVKFQKKGD YPLFGCKYDF NLEGVVDVPE FLVYFPLLNE MAKKYNMKLV YKKTFLEFYE EKIKNNENKM LLKRMQALEP YPANESSKLV SEKVDDYEHA AKYMKNSQVR LPLGTLSKSE WEATSIYLVF AFEKQQ