Recombinant human Tollip protein

TOLLIP, also known as Toll interacting protein, is an inhibitory adaptor protein within Toll-like receptors. The TLR pathway is a part of the innate immune system that recognizes structurally conserved molecular patterns of microbial pathogens, leading to an inflammatory immune response. Negative regulation of TLR signaling by TOLLIP may limit the production of proinflammatory mediators during inflammation and infection. Also, it forms a complex with Tom1 to regulate endosomal trafficking of ubiquitinated proteins. Recombinant human TOLLIP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Toll interacting protein, FLJ33531, IL-1RAcPIP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

32.4 kDa (294aa) confirmed by MALDI-TOF

Additionnal Information

Tollip, Toll interacting protein, FLJ33531, IL-1RAcPIP, ATGP1067-10 µg, ATGP1067-20 µg, ATGP1067-50 µg, ATGP1067-100 µg, ATGP1067-250 µg, ATGP1067-500 µg, ATGP1067-1 mg, ATGP1067-10, ATGP1067-20, ATGP1067-50, ATGP1067-100, ATGP1067-250, ATGP1067-500, ATGP1067-1

References & Citations

Burns K. et al. (2000) Nat. Cell Biol. 2: 346-351.; ; Zhang G. et al. (2002) J. Biol. Chem. 277: 7059-7065.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.2M NaCl,2mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_061882.2

Uniprot Accession Number

Q9H0E2

Species

Human

AA Sequence

MATTVSTQRG PVYIGELPQD FLRITPTQQQ RQVQLDAQAA QQLQYGGAVG TVGRLNITVV QAKLAKNYGM TRMDPYCRLR LGYAVYETPT AHNGAKNPRW NKVIHCTVPP GVDSFYLEIF DERAFSMDDR IAWTHITIPE SLRQGKVEDK WYSLSGRQGD DKEGMINLVM SYALLPAAMV MPPQPVVLMP TVYQQGVGYV PITGMPAVCS PGMVPVALPP AAVNAQPRCS EEDLKAIQDM FPNMDQEVIR SVLEAQRGNK DAAINSLLQM GEEP