Recombinant human DNAL4 protein
DNAL4 is a component of the dynein motor complex in cells which converts the chemical energy contained in ATP into the mechanical energy of movement. Dynein transports various cellular cargo by 'walking' along cytoskeletal microtubules towards the minus-end of the microtubule, which is usually oriented towards the cell center. Recombinant human DNAL4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Dynein light chain 4 axonemal, Dynein light chain 4, axonemal, PIG27
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
14.1 kDa (125aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Rual JF. et al. (2005) Nature. 437: 1173-8.; ; Iwasaki M. et al. (2005) Blood. 105 : 784-93.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_005731
Uniprot Accession Number
O96015
Species
Human
AA Sequence
MGETEGKKDE ADYKRLQTFP LVRHSDMPEE MRVETMELCV TACEKFSNNN ESAAKMIKET MDKKFGSSWH VVIGEGFGFE ITHEVKNLLY LYFGGTLAVC VWKCS
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