Recombinant human PPCS protein

PPCS, also known as phosphopantothenate--cysteine ligase, catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. Recombinant human PPCS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Phosphopantothenate--cysteine ligase, COAB, PPC synthetase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

36.1 kDa (331aa) confirmed by MALDI-TOF

Additionnal Information

PPCS, Phosphopantothenate--cysteine ligase, COAB, PPC synthetase, ATGP1064-10 µg, ATGP1064-20 µg, ATGP1064-50 µg, ATGP1064-100 µg, ATGP1064-250 µg, ATGP1064-500 µg, ATGP1064-1 mg, ATGP1064-10, ATGP1064-20, ATGP1064-50, ATGP1064-100, ATGP1064-250, ATGP1064-500, ATGP1064-1

References & Citations

Manoj N. et al. (2003) Structure. 11 : 927-936.; ; Christina Spry. et al. (2009) J Biol Chem. 284: 24904-24913.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_078940

Uniprot Accession Number

Q9HAB8

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAEMDPVAEF PQPPGAARWA EVMARFAARL GAQGRRVVLV TSGGTKVPLE ARPVRFLDNF SSGRRGATSA EAFLAAGYGV LFLYRARSAF PYAHRFPPQT WLSALRPSGP ALSGLLSLEA EENALPGFAE ALRSYQEAAA AGTFLAVEFT TLADYLHLLQ AAAQALNPLG PSAMFYLAAA VSDFYVPVSE MPEHKIQSSG GPLQITMKMV PKLLSPLVKD WAPKAFIISF KLETDPAIVI NRARKALEIY QHQVVVANIL ESRQSFVFIV TKDSETKLLL SEEEIEKGVE IEEKIVDNLQ SRHTAFIGDR N