Recombinant E.coli tpx protein

TPX, also known as lipid hydroperoxide peroxidase, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein may play an important role in thioredoxin peroxidase activity. Recombinant E. coli TPX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Thiol peroxidase, Peroxiredoxin tpx , Scavengase p20, Thioredoxin peroxidase, Thioredoxin-dependent peroxiredoxin, yzzJ

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

19.9 kDa (188aa) confirmed by MALDI-TOF

Additionnal Information

TPX, Lipid hydroperoxide peroxidase., ECK1320, JW1317, yzzJ, ATGP1057-10 µg, ATGP1057-20 µg, ATGP1057-50 µg, ATGP1057-100 µg, ATGP1057-250 µg, ATGP1057-500 µg, ATGP1057-1 mg, ATGP1057-10, ATGP1057-20, ATGP1057-50, ATGP1057-100, ATGP1057-250, ATGP1057-500, ATGP1057-1

References & Citations

Hambarde S., et al. (2010) Bioin formation. 4 (9) :399-404. ; ; Touchon M., et al. (2009) PLoS Genet. 5 (1) :e1000344.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_415840

Uniprot Accession Number

P0A862

Species

E.coli

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSQTVHFQGN PVTVANSIPQ AGSKAQTFTL VAKDLSDVTL GQFAGKRKVL NIFPSIDTGV CAASVRKFNQ LATEIDNTVV LCISADLPFA QSRFCGAEGL NNVITLSTFR NAEFLQAYGV AIADGPLKGL AARAVVVIDE NDNVIFSQLV DEITTEPDYE AALAVLKA