Recombinant human RhoB protein
Rho-related GTP-binding protein RhoB, also known as RhoB, is a member of the Rho GTP-binding protein family. RhoB mediates apoptosis in neoplastically transformed cells after DNA damage. It plays a negative role in tumorigenesis as deletion causes tumor formation. Recombinant human RhoB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Rho-related GTP-binding protein RhoB, ARH6, ARHB, MST081, MSTP081, RHOH6, Ras homolog family member B, Rho cDNA clone 6, Oncogene RHO H6
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
23.9 kDa (213aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Wennerberg K., et al. (2004) Journal of cell science 117 (Pt 8) : 1301-12.; ; Wherlock M., et al (2004) J. Cell Sci. 117:3221-3231
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 0.2mM NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_004031.1
Uniprot Accession Number
P62745
Species
Human
AA Sequence
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