Recombinant human RhoB protein

Rho-related GTP-binding protein RhoB, also known as RhoB, is a member of the Rho GTP-binding protein family. RhoB mediates apoptosis in neoplastically transformed cells after DNA damage. It plays a negative role in tumorigenesis as deletion causes tumor formation. Recombinant human RhoB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Rho-related GTP-binding protein RhoB, ARH6, ARHB, MST081, MSTP081, RHOH6, Ras homolog family member B, Rho cDNA clone 6, Oncogene RHO H6

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.9 kDa (213aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

RhoB, Rho-related GTP-binding protein RhoB, ARH6, ARHB, MST081, MSTP081, RHOH6, ATGP1056-10 µg, ATGP1056-20 µg, ATGP1056-50 µg, ATGP1056-100 µg, ATGP1056-250 µg, ATGP1056-500 µg, ATGP1056-1 mg, ATGP1056-10, ATGP1056-20, ATGP1056-50, ATGP1056-100, ATGP1056-250, ATGP1056-500, ATGP1056-1

References & Citations

Wennerberg K., et al. (2004) Journal of cell science 117 (Pt 8) : 1301-12.; ; Wherlock M., et al (2004) J. Cell Sci. 117:3221-3231

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 0.2mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004031.1

Uniprot Accession Number

P62745

Species

Human

AA Sequence

MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCC