Recombinant human SPSB1 protein
SPRY domain-containing SOCS box protein 1 (SPSB1), also known as SSB1, is a member of the SOCS box protein subfamily. This protein contains a central SPRY domain and a C-terminal SOCS box. Although some of the SOCS protein subfamilies function as adaptors for a large family of ubiquitin-protein isopeptide ligases to regulate certain signaling pathways, the function of the SSB subfamily remains to be determined. SPSB1 may play an important role in enhancing the HGF-induced Erk-Elk-1-SRE pathway. Over expression of SPSB1 exhibited no effect on the basal level or epidermal growth factor-induced SRE-luciferase activity. Recombinant human SPSB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
SPRY domain-containing SOCS box protein 1, SSB-1, SSB1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
26.1 kDa (231aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Woo J S., et al. (2006) Equb. 25 (6) :1353-63.; ; Wang D., et al. (2005) J Biol Chem. 280 (16) :16393-401.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
Scientific Category
Ubiquitination
NCBI Accession Number
NP_079382
Uniprot Accession Number
Q96BD6
Species
Human
AA Sequence
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