Recombinant e.coli glsA1 protein

GlsA1 (glutaminase1) belongs to the large superfamily of serine-dependent beta-lactamases and penicillin-binding proteins. They are present in most bacteria and eukaryotes and catalyze the hydrolytic deamidation of l-glutamine to l-glutamate and free NH4+. Recombinant E. coli glsA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glutaminase1, ybaS

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

35.0 kDa (330aa) confirmed by MALDI-TOF

Additionnal Information

GlsA1, Glutaminase1, ybaS, ATGP1053-10 µg, ATGP1053-20 µg, ATGP1053-50 µg, ATGP1053-100 µg, ATGP1053-250 µg, ATGP1053-500 µg, ATGP1053-1 mg, ATGP1053-10, ATGP1053-20, ATGP1053-50, ATGP1053-100, ATGP1053-250, ATGP1053-500, ATGP1053-1

References & Citations

Brown G., et al. (2008) Biochemistry. 47 (21) :5724-35. ; ; Nandakumar R., et al. (2003) J. Mol. Catal. B: Enzymes. 23:87-100.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_415018.1

Uniprot Accession Number

P77454

Species

E.coli

AA Sequence

MLDANKLQQA VDQAYTQFHS LNGGQNADYI PFLANVPGQL AAVAIVTCDG NVYSAGDSDY RFALESISKV CTLALALEDV GPQAVQDKIG ADPTGLPFNS VIALELHGGK PLSPLVNAGA IATTSLINAE NVEQRWQRIL HIQQQLAGEQ VALSDEVNQS EQTTNFHNRA IAWLLYSAGY LYCDAMEACD VYTRQCSTLL NTIELATLGA TLAAGGVNPL THKRVLQADN VPYILAEMMM EGLYGRSGDW AYRVGLPGKS GVGGGILAVV PGVMGIAAFS PPLDEDGNSV RGQKMVASVA KQLGYNVFKG