Recombinant e.coli glsA1 protein
GlsA1 (glutaminase1) belongs to the large superfamily of serine-dependent beta-lactamases and penicillin-binding proteins. They are present in most bacteria and eukaryotes and catalyze the hydrolytic deamidation of l-glutamine to l-glutamate and free NH4+. Recombinant E. coli glsA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glutaminase1, ybaS
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
35.0 kDa (330aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Brown G., et al. (2008) Biochemistry. 47 (21) :5724-35. ; ; Nandakumar R., et al. (2003) J. Mol. Catal. B: Enzymes. 23:87-100.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_415018.1
Uniprot Accession Number
P77454
Species
E.coli
AA Sequence
Frequently Asked Questions
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