Recombinant e.coli nth protein
Endonuclease III, also known as nth, is a DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. Recombinant E. coli nth protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Endonuclease III, b1633, JW1625, DNA- (apurinic or apyrimidinic site) lyase, Endonuclease-III
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.7 kDa (231aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Cunningham R.P., et al. (1994) Acad. Sci. 726:215-222; ; Asahara H., et al. (1989) Biochemistry. 28:4444-4449
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_416150
Uniprot Accession Number
P0AB83
Species
E.coli
AA Sequence
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