Recombinant human ETFB protein
ETF (electron transfer flavoprotein) is a heterodimer of an alpha and beta subunit. The ETFB protein is electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Recombinant human ETFB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Electron transfer flavoprotein subunit beta, FP585, MADD
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
30 kDa (275aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Colombo I., et al. (1994) Hum. Mol. Genet. 3:429-435; ; Bross P., et al. (1999) Mol. Genet. Metab. 67:138-147
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_001976
Uniprot Accession Number
P38117
Species
Human
AA Sequence
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